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N1-Methylguanosine-5\'-Triphosphate kinase Enzymatic ass
2019-09-02
Enzymatic assay. The usual in vitro test for the measure of the activity of DbH involves ascorbate as a cosubstrate (e.g., 5mM) and tyramine as a substrate (e.g., 10mM). When using N-aryl-N′-hydroxyguanidines instead of ascorbate, the hydroxylase activity of DbH was measured by HPLC as the amounts o
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br Introduction Estrogens play an important role in
2019-09-02
Introduction Estrogens play an important role in the scr 11 (Arevalo et al., 2015). During several decades, estrogen withdrawal in women has been associated with cognitive impairment, increased Alzheimer\'s disease (AD) risk (Pike, 2017) and with psychiatric disorders such as schizophrenia (Kulk
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PET CT static images of F CJ acquired at h
2019-09-02
PET/CT static images of F-CJ-042794 acquired at 1h p.i. from LNCaP tumour-bearing mice with/without co-injection of non-radioactive CJ-042794 (50μg) are shown in . The LNCaP tumours were clearly visualized in PET images with a moderate tumour-to-background contrast. High radioactivity accumulation w
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Three DDR binding sites have been mapped
2019-09-02
Three DDR2 binding sites have been mapped on the collagen triple helix by us for collagen type 1 and by others using the collagen toolkit for collagen type 2. All of the three reported binding sequences are conserved in the α1 chain of collagen types 1, 2 and 3. The central motif sequence GARGQAGVMG
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Table presents results for estimation of Eq using ACTR
2019-09-02
Table 4 presents results for estimation of Eq. (1) using ACTR and SUWE to compare the incidence of attempting the exam between programs. We find alumni are more likely to attempt the exam if they APETx2 do not hold another professional certification, have worked for an accounting firm, have a highe
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Hence what is proposed here
2019-09-02
Hence, what is proposed here is a technique highly used in reliability engineering: Software Rejuvenation[4], [21], a technique of proactive fault tolerance in which the system is periodically reboot to clean the memory. In fact, it is well known that most critical SW failures are transient. These m
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Why are Tfh cell dynamics
2019-08-30
Why are Tfh cell dynamics so fundamentally different in naive and immune animals? Initially, GC open a bun shop must pass stringent affinity and specificity checkpoints to ensure only high-affinity non-self-reactive cells are selected. Therefore, restricting primary Tfh cells with the greatest helpe
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In all available E E
2019-08-30
In all available E2:E3 structures, the RING-type domain binds the E2 on a surface that is remote from the active site Cys (and therefore from the ubiquitin thioester) (Fig. 2). The non-contiguous E3-binding and active sites on the E2 imply that the role played by a RING to facilitate ubiquitin trans
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In essential hypertensive patients it is known that the CB
2019-08-30
In essential hypertensive patients, it is known that the CB is enlarged (Habeck, 1986). In addition to morphological changes in the CB, hyperventilation was reported in essential hypertensive patients at resting conditions (Trzebski et al., 1982). Furthermore, CB is innervated by postganglionic symp
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A related cell based approach was used to
2019-08-30
A related, cell-based approach was used to test the functionality of the various HIV Rev fusions, but in a trans-complementation assay using 293T producers transfected with both VSV G and a Rev-deficient HIV reporter vector encoding both FFLUC and bsd (Fig. 2C). As expected, the non-fused, full-leng
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TR provides requirements for qualification of commercial PLC
2019-08-30
TR-107330 (1996) provides requirements for qualification of commercial PLC. It uses the criteria of NUREG/CR-6421. EPRI TR-104159 (1995) includes experience about dedicating COTS SW for PLC-based digital systems such as DAFAS (Diverse Auxiliary Feedwater Actuation System) and Emergency Diesel Bus Lo
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CK comprises a family of
2019-08-30
CK1 comprises a family of serine/threonine protein kinases, that includes different CK1 isoforms namely α, β, δ, ε and γ their various splice variants [56]. It was reported that the pharmacological inhibitor IC261 shows an order of magnitude of higher selectivity for CK1δ or CK1ε isoforms (IC50 of 1
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br Experimental Procedures br Author
2019-08-30
Experimental Procedures Author Contributions Acknowledgments We thank the Mouse Modeling, Integrated Microscopy, and FACS Facilities of IGB-CNR, Naples. Dr. Laura Pisapia is acknowledged for flow cytometry analyses. We are grateful to Prof. Chris Ponting and George A. Calin for insightful s
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br Author Contributions br Acknowledgments This work was sup
2019-08-30
Author Contributions Acknowledgments This work was supported, in part, by a grant from the National Cancer Institute of the National Institutes of Health under Grant U01CA202241 and National Institutes of Health under Grant R01NS083856. Introduction The Eph (erythropoietin-producing human
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br O GlcNAc transferase OGT belongs
2019-08-30
O-GlcNAc transferase OGT belongs to the metal-independent GT-B superfamily of glycosyltransferases, which has been well-reviewed previously [14,15]. OGT is an essential gene encoded on the X-chromosome, and it has two main regions: a long N-terminal tetratricopeptide repeat (TPR) region and a C-t
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